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KMID : 0811720070110040149
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Korean Journal of Physiology & Pharmacology
2007 Volume.11 No. 4 p.149 ~ p.154
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Identification of Differentially Expressed Genes in Murine Hippocampus by Modulation of Nitric Oxide in Kainic Acid-induced Neurotoxic Animal Model
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Suh Yo-Ahn
Lim So-Young
Kim Sung-Soo
Lee Hee-Jae
Kwon O-Min
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Abstract
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Kainic acid (KA) causes neurodegeneration, but no consensus has been reached concerning its mechanism. Nitric oxide may be a regulator of the mechanism. We identified differentially expressed genes in the hippocampus of mice treated with kainic acid, together with or without L-NAME, a nonselective nitric oxide synthase inhibitor, using a new differential display PCR method based on annealing control primers. Eight genes were identified, including clathrin light polypeptide, TATA element modulatory factor 1, neurexin III, ND4, ATPase, H+ transporting, V1 subunit E isoform 1, and N-myc downstream regulated gene 2. Although the functions of these genes and their products remain to be determined, their identification provides insight into the molecular mechanism(s) involved in KA-induced neuronal cell death in the hippocampal CA3 area.
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KEYWORD
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Excitotoxicity, Kainic acid, L-NAME, Differential display PCR, Hippocampus
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